4RX2
A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Beta-lactamase TEM | polymer | 263 | 28882.9 | 8 | UniProt (P62593) Pfam (PF00144) Pfam (PF13354) | Escherichia coli | IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2 |
| 2 | I, J, K, L, M... (A, B, C, D, E...) | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
| 3 | Q, R, S, T, U... (A, B, C, D, E...) | water | water | 18.0 | 817 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 26 - 290 (UniProt: P62593)
| PDB | External Database | Details |
|---|---|---|
| Tyr 165 | Trp 163 | engineered mutation |
| Tyr 166 | Glu 164 | engineered mutation |
| Gly 167 | Pro 165 | engineered mutation |
| Thr 182 | Met 180 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 231062.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 768.5 | |
| All* | Total formula weight | 231831.4 |
