4RWG

Crystal structure of the CLR:RAMP1 extracellular domain heterodimer with bound high affinity CGRP analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Maltose-binding periplasmic protein, Receptor activity-modifying protein 1, Calcitonin gene-related peptide type 1 receptor fusion protein	polymer	593	66304.6	3	UniProt (P0AEX9) UniProt (O60894) UniProt (Q16602) Pfam (PF01547) Pfam (PF04901) Pfam (PF02793) In PDB	Escherichia coli
2	D, E, F	CGRP analog	polymer	12	1194.4	3		synthetic construct
3	G, H, I	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	3	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5		water	water		18.0	70	Chemie (HOH)

Sequence modifications

A, B, C: 2 - 374 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine

A, B, C: 1024 - 1108 (UniProt: O60894)
A, B, C: 2031 - 2114 (UniProt: Q16602)

PDB	External Database	Details
Arg 1109	-	linker
Ala 1110	-	linker
Val 2019	-	expression tag
Gly 2020	-	expression tag
Ser 2021	-	expression tag
Ala 2022	-	expression tag
Gly 2023	-	expression tag
Ser 2024	-	expression tag
Ala 2025	-	expression tag
Gly 2026	-	expression tag
Ser 2027	-	expression tag
Ala 2028	-	expression tag
Glu 2029	-	expression tag
Asp 2030	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	202496.8
Branched	Number of molecules	3
	Total formula weight	1026.9
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	203548.0

*Water molecules are not included.