4RTK

Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with SAH and with DNA Containing Distal Pap Regulon Sequence

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA adenine methylase	polymer	298	34331.0	1	UniProt (H0Q7C9) Pfam (PF02086) UniProt (by SIFTS) (P0AEE8) In PDB	Escherichia coli
2	F	DNA (5'-D(*TP*CP*TP*AP*AP*AP*GP*AP*TP*CP*G)-3')	polymer	11	3357.2	1		synthetic construct
3	G	DNA (5'-D(*AP*CP*GP*AP*TP*CP*TP*TP*TP*AP*G)-3')	polymer	11	3348.2	1		synthetic construct
4	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	99	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: H0Q7C9)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 175	Ala 175	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	41036.4
Non-Polymers*	Number of molecules	2
	Total formula weight	476.5
All*	Total formula weight	41512.9

*Water molecules are not included.