4RTJ

A non-cognate complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with DNA and Sinefungin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA adenine methylase	polymer	298	34331.0	1	UniProt (H0Q7C9) Pfam (PF02086) UniProt (by SIFTS) (P0AEE8)	Escherichia coli
2	B, C (F, G)	DNA (5'-D(*TP*CP*TP*AP*GP*AP*TP*CP*TP*AP*GP*A)-3')	polymer	12	3661.4	2		synthetic construct
3	D (A)	SINEFUNGIN	non-polymer		381.4	1	Chemie (SFG) PubChem (176612030) PubChem (177116271) PubChem (25246236) PubChem (456189) PubChem (46875078) PubChem (46936257) PubChem (4065234) PubChem (70602121) PubChem (65482) PubChem (6917665) PubChem (70789212) PubChem (92403972) PubChem (93287270) PubChem (93287272) PubChem (93475589) PubChem (12771337) PubChem (93287274) PubChem (12771338) PubChem (131971033) PubChem (131972810) PubChem (138626357) PubChem (156562738) PubChem (162866891) PubChem (163339130) PubChem (168280605)
4	E (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	F, G, H (A, F, G)	water	water		18.0	97	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: H0Q7C9)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 175	Ala 175	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	41653.8
Non-Polymers*	Number of molecules	2
	Total formula weight	443.5
All*	Total formula weight	42097.2

*Water molecules are not included.