4RSN

Crystal structure of the E267V mutant of cytochrome P450 BM3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Bifunctional P-450/NADPH-P450 reductase	polymer	460	52644.1	2	UniProt (P14779) Pfam (PF00067) In PDB	Bacillus megaterium	Cytochrome P450(BM-3), Cytochrome P450BM-3, Cytochrome P450 102, NADPH--cytochrome P450 reductase
2	A, B	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3		water	water		18.0	11	Chemie (HOH)

Sequence modifications

A, B: 0 - 455 (UniProt: P14779)

PDB	External Database	Details
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Leu 47	Arg 48	engineered mutation
Phe 51	Tyr 52	engineered mutation
Val 87	Phe 88	engineered mutation
Val 267	Glu 268	engineered mutation
Asp 315	Gly 316	conflict
Lys 344	Glu 345	conflict
Pro 401	Ile 402	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	105288.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1233.0
All*	Total formula weight	106521.2

*Water molecules are not included.