4RQ5

Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine (8-oxoG)and dATP soaked with MgCl2 for 60 s

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase beta	polymer	343	39258.8	1	UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (human)
2	B (D)	DNA (5'-D(P*GP*TP*CP*GP*G)-3')	polymer	5	1536.0	1		synthetic construct	5-mer phosphorylated Downstream Primer
3	C (P)	DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*CP*A)-3')	polymer	11	3374.2	1		synthetic construct	10-mer Primer
4	D (T)	DNA (5'-D(*CP*CP*GP*AP*CP*(8OG)P*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')	polymer	16	4885.2	1		synthetic construct	16-mer Template
5	E, F, O (A, P)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG) PubChem (888)
6	G, H (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA) PubChem (923)
7	I, J (A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT) PubChem (175)
8	K, L (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL) PubChem (312)
9	M (A)	PYROPHOSPHATE	non-polymer		178.0	1	Chemie (PPV) PubChem (1023)
10	N (A)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP) PubChem (44119995)
11	P, Q, R, S (A, D, P, T)	water	water		18.0	76	Chemie (HOH)

Sequence modifications

A: 1 - 335 (UniProt: P06746)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49054.2
Non-Polymers*	Number of molecules	11
	Total formula weight	977.0
All*	Total formula weight	50031.2

*Water molecules are not included.