4RQ0
Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine (8-oxoG)and dCTP soaked with MgCl2 for 80 s
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase beta | polymer | 343 | 39258.8 | 1 | UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB | Homo sapiens (human) | |
2 | D | DNA (5'-D(P*GP*TP*CP*GP*G)-3') | polymer | 5 | 1536.0 | 1 | synthetic construct | 5-mer phosphorylated Downstream Primer | |
3 | P | DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*CP*C)-3') | polymer | 11 | 3350.2 | 1 | synthetic construct | 11-mer Primer | |
4 | T | DNA (5'-D(*CP*CP*GP*AP*CP*(8OG)P*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') | polymer | 16 | 4885.2 | 1 | synthetic construct | 16-mer Template | |
5 | A | PYROPHOSPHATE | non-polymer | 178.0 | 1 | Chemie (PPV) | |||
6 | A | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DCP) | |||
7 | A, P | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
8 | A | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
9 | A | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
10 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
11 | water | water | 18.0 | 126 | Chemie (HOH) |
Sequence modifications
A: 1 - 335 (UniProt: P06746)
PDB | External Database | Details |
---|---|---|
Met -1 | - | expression tag |
Gly 0 | - | expression tag |
His 336 | - | expression tag |
His 337 | - | expression tag |
His 338 | - | expression tag |
His 339 | - | expression tag |
His 340 | - | expression tag |
His 341 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 49030.2 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 892.7 | |
All* | Total formula weight | 49922.8 |