4RPE

Crystal Structure of Variant G186E from Pseudomonas Aeruginosa Lipoxygenase 2 at 1.60A (C2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Linoleate 9/13-lipoxygenase	polymer	688	75125.6	1	UniProt (Q8RNT4) Pfam (PF00305)	Pseudomonas aeruginosa	Oleate 10S-lipoxygenase, 15S-Lipoxygenase
2	B (A)	FE (II) ION	non-polymer		55.8	1	Chemie (FE2)
3	C (A)	(2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate	non-polymer		692.0	1	Chemie (8PE)
4	D (A)	water	water		18.0	478	Chemie (HOH)

Sequence modifications

A: 19 - 685 (UniProt: Q8RNT4)

PDB	External Database	Details
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Leu 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Arg 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Met 18	-	expression tag
Glu 186	Gly 186	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	75125.6
Non-Polymers*	Number of molecules	2
	Total formula weight	747.8
All*	Total formula weight	75873.4

*Water molecules are not included.