4RM1
The crystal structure of Y333Q mutant pyridoxal-dependent decarboxylase from Sphaerobacter thermophilus DSM 20745
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D (B, A, C, D) | Pyridoxal-dependent decarboxylase | polymer | 486 | 53080.4 | 4 | UniProt (D1C7D8) Pfam (PF00282) | Sphaerobacter thermophilus DSM 20745 | |
2 | E, M (B, C) | 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE | non-polymer | 248.2 | 2 | Chemie (PMP) | |||
3 | F, G, H, J, K... (B, A) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
4 | I, N (B, D) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
5 | O, P, Q, R (B, A, C, D) | water | water | 18.0 | 1116 | Chemie (HOH) |
Sequence modifications
B, A, C, D: 1 - 483 (UniProt: D1C7D8)
PDB | External Database | Details |
---|---|---|
Ser -2 | - | expression tag |
Asn -1 | - | expression tag |
Ala 0 | - | expression tag |
Gln 333 | Tyr 333 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 212321.8 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1119.8 | |
All* | Total formula weight | 213441.6 |