4RI8

FAN1 Nuclease bound to 5' phosphorylated p(dG)/3'(dT-dT-dT-dT) double flap DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fanconi-associated nuclease 1	polymer	651	73926.0	2	UniProt (Q9Y2M0) Pfam (PF21315) Pfam (PF21169) Pfam (PF21170) Pfam (PF08774)	Homo sapiens (human)	FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15
2	C, F (E, H)	DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*TP*T)-3')	polymer	14	4316.8	2
3	D, G (F, I)	DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3')	polymer	8	2507.7	2
4	E, H (G, J)	DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3')	polymer	19	5718.7	2
5	I, J (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

A, B: 370 - 1017 (UniProt: Q9Y2M0)

PDB	External Database	Details
Gly 358	-	expression tag
Ala 359	-	expression tag
His 360	-	expression tag
Met 361	-	expression tag
Thr 362	-	expression tag
Arg 363	-	expression tag
Asn 364	-	expression tag
Gly 365	-	expression tag
Pro 366	-	expression tag
Gly 367	-	expression tag
Gln 368	-	expression tag
Thr 369	-	expression tag
Ala 487	Val 487	engineered mutation
-	Cys 510	deletion
-	Thr 511	deletion
-	Trp 512	deletion
-	Gly 513	deletion
-	Lys 514	deletion
-	Asn 515	deletion
-	Lys 516	deletion
-	Pro 517	deletion
-	Gly 518	deletion

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	172938.4
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	173018.6

*Water molecules are not included.