4RHQ

Crystal structure of T. brucei arginase-like protein double mutant S149D/S153D

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Arginase	polymer	351	38788.3	3	UniProt (Q581Y0) Pfam (PF00491)	Trypanosoma brucei brucei
2	D, H (A, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	E, F, G, I (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
4	J, K, L (A, B, C)	water	water		18.0	163	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 331 (UniProt: Q581Y0)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 149	Ser 149	engineered mutation
Asp 153	Ser 153	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	116364.8
Non-Polymers*	Number of molecules	6
	Total formula weight	432.5
All*	Total formula weight	116797.3

*Water molecules are not included.