4RHM

Crystal structure of T. brucei arginase-like protein quadruple mutant S149D/R151H/S153D/S226D

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Arginase	polymer	351	38797.2	3	UniProt (Q581Y0) Pfam (PF00491)	Trypanosoma brucei brucei
2	D, F, H, K, M... (A, B, C)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
3	E, I, N (A, B, C)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
4	G, J, L (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	P, Q, R (A, B, C)	water	water		18.0	129	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 331 (UniProt: Q581Y0)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 149	Ser 149	engineered mutation
His 151	Arg 151	engineered mutation
Asp 153	Ser 153	engineered mutation
Asp 226	Ser 226	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	116391.7
Non-Polymers*	Number of molecules	12
	Total formula weight	903.6
All*	Total formula weight	117295.3

*Water molecules are not included.