4RFV

Structure of the Mycobacterium tuberculosis APS kinase CysC Cys556Ala mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bifunctional enzyme CysN/CysC	polymer	193	21278.1	2	UniProt (P9WNM5) Pfam (PF01583)	Mycobacterium tuberculosis	Sulfate adenylyltransferase subunit 1, ATP-sulfurylase large subunit, Sulfate adenylate transferase, SAT, Adenylyl-sulfate kinase, APS kinase, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
2	C (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
3	D, E (A, B)	water	water		18.0	254	Chemie (HOH)

Sequence modifications

A, B: 424 - 612 (UniProt: P9WNM5)

PDB	External Database	Details
Gly 420	-	expression tag
Ser 421	-	expression tag
His 422	-	expression tag
Met 423	-	expression tag
Ser 556	Cys 556	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	42556.2
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	42651.1

*Water molecules are not included.