4RDM

Crystal structure of R.NgoAVII restriction endonuclease B3 domain with cognate DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Restriction endonuclease R.NgoVII	polymer	178	20666.1	2	UniProt (Q5F9M9) Pfam (PF20731) In PDB	Neisseria gonorrhoeae
2	C, E	DNA (5'-D(*CP*CP*TP*AP*AP*GP*CP*GP*GP*CP*AP*AP*TP*CP*C)-3)'	polymer	15	4539.0	2
3	D, F	DNA (5'-D(*GP*GP*GP*AP*TP*TP*GP*CP*CP*GP*CP*TP*TP*AP*G)-3)'	polymer	15	4626.0	2
4	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5		water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, B: 179 - 345 (UniProt: Q5F9M9)

PDB	External Database	Details
Met 178	-	initiating methionine
Ser 346	-	expression tag
Gly 347	-	expression tag
Gly 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
Gly 355	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	59662.0
Non-Polymers*	Number of molecules	1
	Total formula weight	96.1
All*	Total formula weight	59758.1

*Water molecules are not included.