4R6D

Modified mTFP* for enhanced metal binding: co-crystallization with CuCl2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	GFP-like fluorescent chromoprotein cFP484	polymer	217	24825.1	1	UniProt (Q9U6Y3) Pfam (PF01353) In PDB	Clavularia sp. (Brown star polyp)
2	A	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)
3		water	water		18.0	310	Chemie (HOH)

Sequence modifications

A: 2 - 216 (UniProt: Q9U6Y3)

PDB	External Database	Details
Ser 1	-	expression tag
Tyr 21	His 63	engineered mutation
Asn 38	His 80	engineered mutation
Ile 40	Leu 82	engineered mutation
Thr 58	Ser 100	engineered mutation
Thr 59	Asn 101	engineered mutation
Pia 62	Gln 104	chromophore
Pia 62	Tyr 105	chromophore
Pia 62	Gly 106	chromophore
Phe 68	Leu 110	engineered mutation
Pro 76	Ala 118	engineered mutation
Asn 77	Asp 119	engineered mutation
Leu 109	Met 151	engineered mutation
Tyr 119	Arg 161	engineered mutation
Leu 120	Phe 162	engineered mutation
Lys 121	Asp 163	engineered mutation
Glu 123	Met 165	engineered mutation
Thr 137	Leu 179	engineered mutation
Gly 138	Lys 180	engineered mutation
Asp 140	Glu 182	engineered mutation
Ala 141	Pro 183	engineered mutation
Arg 145	Ile 187	engineered mutation
Lys 154	Val 196	engineered mutation
Val 157	Ile 199	engineered mutation
Lys 158	Ser 200	engineered mutation
Lys 160	Ser 202	engineered mutation
Tyr 168	His 210	engineered mutation
Val 171	Cys 213	engineered mutation
Thr 175	Ser 217	engineered mutation
Arg 178	Lys 220	engineered mutation
Ala 182	Val 224	engineered mutation
Cys 197	Ile 239	engineered mutation
His 204	Tyr 246	engineered mutation
Val 209	Leu 251	engineered mutation
Ser 212	Asn 254	engineered mutation
Asn 217	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	24825.1
Non-Polymers*	Number of molecules	2
	Total formula weight	127.1
All*	Total formula weight	24952.2

*Water molecules are not included.