4R66
Ternary complex crystal structure of E295K mutant of DNA polymerase Beta
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase beta | polymer | 335 | 38241.7 | 1 | UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (human) | |
| 2 | B (T) | DNA (5'-D(*CP*CP*GP*AP*CP*AP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') | polymer | 16 | 4853.2 | 1 | Synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3') | polymer | 10 | 3061.0 | 1 | Synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*GP*TP*CP*GP*G)-3') | polymer | 5 | 1536.0 | 1 | Synthetic construct | ||
| 5 | E (A) | 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DUP) | |||
| 6 | F, G, M, N (A, T, D) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 7 | H, I (A) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 8 | J, K, L (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 9 | O, P, Q, R (A, T, P, D) | water | water | 18.0 | 408 | Chemie (HOH) |
Sequence modifications
A: 1 - 335 (UniProt: P06746)
| PDB | External Database | Details |
|---|---|---|
| Lys 295 | Glu 295 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 47691.9 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 839.2 | |
| All* | Total formula weight | 48531.2 |
