4QRV
Crystal structure of I86F mutant of papain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Papain | polymer | 363 | 41101.6 | 2 | UniProt (P00784) Pfam (PF08246) Pfam (PF00112) | Carica papaya (mamon) | Papaya proteinase I, PPI |
| 2 | C, E (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 3 | D, F (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 369 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 319 (UniProt: P00784)
| PDB | External Database | Details |
|---|---|---|
| Met -43 | - | expression tag |
| His -42 | - | expression tag |
| His -41 | - | expression tag |
| His -40 | - | expression tag |
| His -39 | - | expression tag |
| His -38 | - | expression tag |
| His -37 | - | expression tag |
| Ser -36 | - | expression tag |
| Ser -35 | - | expression tag |
| Gly -34 | - | expression tag |
| Leu -33 | - | expression tag |
| Val -32 | - | expression tag |
| Pro -31 | - | expression tag |
| Arg -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ser -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Met -26 | - | expression tag |
| Lys -25 | - | expression tag |
| Glu -24 | - | expression tag |
| Thr -23 | - | expression tag |
| Ala -22 | - | expression tag |
| Ala -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Lys -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Glu -17 | - | expression tag |
| Arg -16 | - | expression tag |
| Gln -15 | - | expression tag |
| His -14 | - | expression tag |
| Met -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Pro -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Thr -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Lys -1 | - | expression tag |
| Met 0 | - | expression tag |
| Phe 86 | Ile 112 | engineered mutation |
| Ala 132 | Cys 158 | engineered mutation |
| Ser 139 | Val 165 | engineered mutation |
| Ser 143 | Gly 169 | engineered mutation |
| Arg 281 | Lys 307 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 82203.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 116.9 | |
| All* | Total formula weight | 82320.1 |
