4QLL
Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-glucosidase 7 | polymer | 481 | 54578.3 | 2 | UniProt (Q75I93) Pfam (PF00232) | Oryza sativa Japonica Group (Japanese rice) | Os3bglu7 |
| 2 | C, D, E (C, D, E) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 666.6 | 3 | In PDB BIRD (PRD_900011) GlyTouCan (G00025MO) | beta-cellotetraose | ||
| 3 | F (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 4 | G, I (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | H, J (A, B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 6 | K, L (A, B) | water | water | 18.0 | 839 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 476 (UniProt: Q75I93)
| PDB | External Database | Details |
|---|---|---|
| Ala -4 | - | expression tag |
| Met -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Val 0 | - | expression tag |
| Val 24 | Ala 52 | SEE REMARK 999 |
| Gln 176 | Glu 204 | engineered mutation |
| Ala 187 | Gln 215 | engineered mutation |
| Ala 341 | Tyr 369 | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 109156.7 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1999.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 648.0 | |
| All* | Total formula weight | 111804.4 |
