4Q7U

Crystal structure of photoswitchable fluorescent protein PSmOrange2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	PSmOrange2	polymer	245	28152.9	1	UniProt (D0VWW2) Pfam (PF01353)	Discosoma sp. (Sea anemone)
2	B (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	C (A)	water	water		18.0	211	Chemie (HOH)

Sequence modifications

A: -4 - 231 (UniProt: D0VWW2)

PDB	External Database	Details
Met -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Arg -6	-	expression tag
Ser -5	-	expression tag
His 17	Arg 22	engineered mutation
Thr 21	Ser 26	engineered mutation
His 36	Arg 41	engineered mutation
Leu 64	Gln 69	engineered mutation
Oim 66	Phe 70	chromophore
Oim 66	Gly 71	chromophore
Oim 66	Tyr 72	chromophore
Oim 66	Gly 73	chromophore
Tyr 99	Phe 104	engineered mutation
Met 124	Leu 129	engineered mutation
Arg 162	Lys 167	engineered mutation
Ser 186	Pro 191	engineered mutation
Leu 188	Gln 193	engineered mutation
Ser 217	Ala 222	engineered mutation
Ala 219	Gly 224	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28152.9
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	28245.0

*Water molecules are not included.