4Q7R

Crystal structure of large Stokes shift fluorescent protein LSSmOrange

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	LSSmOrange	polymer	245	28120.7	2	UniProt (D0VWW2) Pfam (PF01353) In PDB	Discosoma sp. (Sea anemone)
2	A, B	ZINC ION	non-polymer		65.4	17	Chemie (ZN)
3	B, A	ACETATE ION	non-polymer		59.0	9	Chemie (ACT)
4		water	water		18.0	301	Chemie (HOH)

Sequence modifications

A, B: -4 - 231 (UniProt: D0VWW2)

PDB	External Database	Details
Met -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Arg -6	-	expression tag
Ser -5	-	expression tag
His 17	Arg 22	engineered mutation
Val 44	Ala 49	engineered mutation
Ofm 66	Phe 70	chromophore
Ofm 66	Gly 71	chromophore
Ofm 66	Tyr 72	chromophore
Ofm 66	Gly 73	chromophore
Leu 83	Phe 88	engineered mutation
Met 143	Trp 148	engineered mutation
Asp 161	Ile 166	engineered mutation
Leu 163	Met 168	engineered mutation
Asp 196	Gly 201	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56241.5
Non-Polymers*	Number of molecules	26
	Total formula weight	1643.3
All*	Total formula weight	57884.8

*Water molecules are not included.