4PZF
Berberine bridge enzyme G164A variant, a reticuline dehydrogenase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Reticuline oxidase | polymer | 538 | 60061.0 | 4 | UniProt (P30986) Pfam (PF01565) Pfam (PF08031) In PDB | Eschscholzia californica (California poppy) | Berberine bridge-forming enzyme, BBE, Tetrahydroprotoberberine synthase |
| 2 | E, F, G, H | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 3 | A, B, C, D | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 4 | Chemie (FAD) | |||
| 4 | A, B, C, D | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 5 | B, C, D | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 6 | C, D | DODECAETHYLENE GLYCOL | non-polymer | 546.6 | 2 | Chemie (12P) | |||
| 7 | water | water | 18.0 | 308 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 538 (UniProt: P30986)
| PDB | External Database | Details |
|---|---|---|
| Glu 22 | Leu 22 | SEE REMARK 999 |
| Ala 23 | Gly 23 | SEE REMARK 999 |
| Ala 164 | Gly 164 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 240244.0 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1697.6 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 5600.6 | |
| All* | Total formula weight | 247542.3 |
