4PXM

The Estrogen Receptor Alpha Ligand Binding Domain D538G Mutant in Complex with Estradiol and a glucocorticoid receptor-interacting protein 1 NR box II peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Estrogen receptor	polymer	271	30888.3	2	UniProt (P03372) Pfam (PF00104)	Homo sapiens (human)	ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
2	C, D (C, D)	Nuclear receptor coactivator 2	polymer	13	1579.9	2	UniProt (Q15596)	Homo sapiens	NCoA-2, Class E basic helix-loop-helix protein 75, bHLHe75, Transcriptional intermediary factor 2, hTIF2
3	E, F (A, B)	ESTRADIOL	non-polymer		272.4	2	Chemie (EST)
4	G, H (A, B)	water	water		18.0	87	Chemie (HOH)

Sequence modifications

A, B: 299 - 554 (UniProt: P03372)

PDB	External Database	Details
Val 284	-	expression tag
Asp 285	-	expression tag
Leu 286	-	expression tag
Gly 287	-	expression tag
Thr 288	-	expression tag
Glu 289	-	expression tag
Asn 290	-	expression tag
Leu 291	-	expression tag
Tyr 292	-	expression tag
Phe 293	-	expression tag
Gln 294	-	expression tag
Ser 295	-	expression tag
Asn 296	-	expression tag
Ala 297	-	expression tag
Met 298	-	expression tag
Gly 538	Asp 538	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	64936.2
Non-Polymers*	Number of molecules	2
	Total formula weight	544.8
All*	Total formula weight	65481.0

*Water molecules are not included.