4PUM
tRNA-Guanine Transglycosylase (TGT) Mutant D156N in Complex with 6-Amino-2-(methylamino)-1H,7H,8H-imidazo[4,5-g]quinazolin-8-one
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Queuine tRNA-ribosyltransferase | polymer | 388 | 43068.8 | 1 | UniProt (P28720) Pfam (PF01702) | Zymomonas mobilis subsp. mobilis | Guanine insertion enzyme, tRNA-guanine transglycosylase |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 4 | E (A) | 6-amino-2-(methylamino)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one | non-polymer | 230.2 | 1 | Chemie (2WU) | |||
| 5 | F (A) | water | water | 18.0 | 219 | Chemie (HOH) |
Sequence modifications
A: 1 - 386 (UniProt: P28720)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Asn 156 | Asp 156 | engineered mutation |
| Lys 312 | Thr 312 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 43068.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 479.8 | |
| All* | Total formula weight | 43548.7 |
