4PNE

Crystal Structure of the [4+2]-Cyclase SpnF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Methyltransferase-like protein	polymer	302	32617.9	2	UniProt (Q9ALM7) Pfam (PF08241)	Saccharopolyspora spinosa
2	C, E (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
3	D, F (A, B)	MALONATE ION	non-polymer		102.0	2	Chemie (MLI)
4	G, H (A, B)	water	water		18.0	222	Chemie (HOH)

Sequence modifications

A, B: 9 - 282 (UniProt: Q9ALM7)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Gln 2	-	expression tag
Arg 3	-	expression tag
Ser 4	-	expression tag
Gly 5	-	expression tag
Ile 6	-	expression tag
Pro 7	-	expression tag
Val 8	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65235.7
Non-Polymers*	Number of molecules	4
	Total formula weight	972.9
All*	Total formula weight	66208.6

*Water molecules are not included.