4PMC
Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Thermonuclease | polymer | 143 | 16189.5 | 1 | UniProt (P00644) Pfam (PF00565) | Staphylococcus aureus | TNase,Micrococcal nuclease,Staphylococcal nuclease |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C (A) | THYMIDINE-3',5'-DIPHOSPHATE | non-polymer | 402.2 | 1 | Chemie (THP) | |||
| 4 | D (A) | water | water | 18.0 | 100 | Chemie (HOH) |
Sequence modifications
A: 1 - 149 (UniProt: P00644)
| PDB | External Database | Details |
|---|---|---|
| Phe 50 | Gly 132 | engineered mutation |
| Asn 51 | Val 133 | engineered mutation |
| Lys 72 | Ile 154 | engineered mutation |
| Lys 74 | Val 156 | engineered mutation |
| Gly 117 | Pro 199 | engineered mutation |
| Leu 124 | His 206 | engineered mutation |
| Ala 128 | Ser 210 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 16189.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 442.3 | |
| All* | Total formula weight | 16631.8 |
