4PIT

Crystal Structure of Banana Lectin H84T bound to dimannose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ripening-associated protein	polymer	149	15611.5	4	UniProt (O22321) Pfam (PF01419)	Musa acuminata (Banana)	lectin
2	E, F, G, H, I (E, F, G, H, I)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose	branched		342.3	5	In PDB BIRD (PRD_900111) GlyTouCan (G53402KW)		2alpha-alpha-mannobiose
3	J, M, O (A, C, D)	alpha-D-mannopyranose	non-polymer		180.2	3	Chemie (MAN)
4	K, L, N, P (A, B, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	Q, R, S, T (A, B, C, D)	water	water		18.0	667	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 141 (UniProt: O22321)

PDB	External Database	Details
Thr 84	His 84	engineered mutation
Leu 142	-	expression tag
Glu 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	62446.1
Branched	Number of molecules	5
	Total formula weight	1711.5
Non-Polymers*	Number of molecules	7
	Total formula weight	908.8
All*	Total formula weight	65066.4

*Water molecules are not included.