4PIO
Ergothioneine-biosynthetic methyltransferase EgtD in complex with N,N-dimethylhistidine and SAH
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Histidine-specific methyltransferase EgtD | polymer | 323 | 35314.7 | 2 | UniProt (A0R5M8) Pfam (PF10017) In PDB | Mycobacterium smegmatis | Histidine-alpha-N-methyltransferase |
| 2 | A, B | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) | |||
| 3 | A, B | N,N-dimethyl-L-histidine | non-polymer | 183.2 | 2 | Chemie (AVI) | |||
| 4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | water | water | 18.0 | 985 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 321 (UniProt: A0R5M8)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 2 | Thr 2 | engineered mutation |
| Thr 29 | Ala 29 | engineered mutation |
| Gln 30 | Pro 30 | engineered mutation |
| Ser 75 | Ala 75 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 70629.4 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1316.5 | |
| All* | Total formula weight | 71945.9 |
