4PIM

Ergothioneine-biosynthetic methyltransferase EgtD, apo form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Histidine-specific methyltransferase EgtD	polymer	323	35517.2	2	UniProt (A0R5M8) Pfam (PF10017)	Mycobacterium smegmatis	Histidine-alpha-N-methyltransferase
2	C, D (A, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
3	E, F (A, B)	water	water		18.0	834	Chemie (HOH)

Sequence modifications

A, B: 1 - 321 (UniProt: A0R5M8)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Ala 2	Thr 2	engineered mutation
Thr 29	Ala 29	engineered mutation
Gln 30	Pro 30	engineered mutation
Ser 75	Ala 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	71034.3
Non-Polymers*	Number of molecules	2
	Total formula weight	118.1
All*	Total formula weight	71152.4

*Water molecules are not included.