4PI8
Crystal structure of catalytic mutant E138A of S. Aureus Autolysin E in complex with disaccharide NAG-NAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Autolysin E | polymer | 228 | 26010.2 | 1 | UniProt (Q99RW6) Pfam (PF01832) UniProt (by SIFTS) (A0A0H3JT72) | Staphylococcus aureus | |
| 2 | B (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-N-acetyl-beta-muramic acid | branched | 496.5 | 1 | In PDB GlyTouCan (G99515FY) | |||
| 3 | C, D, E, F, G... (A) | CHLORIDE ION | non-polymer | 35.5 | 14 | Chemie (CL) | |||
| 4 | N (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | R (A) | water | water | 18.0 | 256 | Chemie (HOH) |
Sequence modifications
A: 35 - 258 (UniProt: Q99RW6)
| PDB | External Database | Details |
|---|---|---|
| Ser 31 | - | expression tag |
| Ala 32 | - | expression tag |
| Ala 33 | - | expression tag |
| Ala 34 | - | expression tag |
| Ala 138 | Glu 138 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 26010.2 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 496.5 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 592.4 | |
| All* | Total formula weight | 27099.1 |
