4PFW

Crystal structure of mannohexaose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.2 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ABC transporter substrate-binding protein	polymer	547	63835.1	2	UniProt (G4FEC0)	Thermotoga maritima	Mannoside ABC transport system,sugar-binding protein
2	C, D (C, D)	beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-alpha-D-mannopyranose-(1-4)-alpha-D-mannopyranose	branched		990.9	2	In PDB GlyTouCan (G32802UG)
3	E, J (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, G, H, I, N (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5	K, L, M (B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	O, P (A, B)	water	water		18.0	528	Chemie (HOH)

Sequence modifications

A, B: 21 - 560 (UniProt: G4FEC0)

PDB	External Database	Details
Met 20	-	initiating methionine
His 561	-	expression tag
His 562	-	expression tag
His 563	-	expression tag
His 564	-	expression tag
His 565	-	expression tag
His 566	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	127670.3
Branched	Number of molecules	2
	Total formula weight	1981.7
Non-Polymers*	Number of molecules	10
	Total formula weight	805.2
All*	Total formula weight	130457.2

*Water molecules are not included.