4PEM
Crystal Structure of S1G mutant of Penicillin G Acylase from Kluyvera citrophila
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, C, E, G) | Penicillin G acylase Alpha | polymer | 286 | 31695.9 | 4 | UniProt (A0A068F6N5) Pfam (PF01804) | Kluyvera cryocrescens | |
| 2 | B, D, F, H (B, D, F, H) | Penicillin G acylase Beta | polymer | 568 | 63254.3 | 4 | UniProt (A0A068F6N5) Pfam (PF01804) | Kluyvera cryocrescens | |
| 3 | I (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | J, K, L, M, N... (A, B, C, D, E...) | water | water | 18.0 | 428 | Chemie (HOH) |
Sequence modifications
A, C, E, G: -19 - 260 (UniProt: A0A068F6N5)
B, D, F, H: 261 - 828 (UniProt: A0A068F6N5)
| PDB | External Database | Details |
|---|---|---|
| Met -25 | - | initiating methionine |
| Lys -24 | - | expression tag |
| Asn -23 | - | expression tag |
| Arg -22 | - | expression tag |
| Asn -21 | - | expression tag |
| Arg -20 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 264 | Ser 284 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 379801.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 379841.1 |
