4PBT
Crystal structure of the M. jannaschii G2 tRNA synthetase variant bound to 4-trans-cyclooctene-amidopheylalanine (Tco-amF)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Tyrosine--tRNA ligase | polymer | 314 | 36113.9 | 1 | UniProt (Q57834) Pfam (PF00579) In PDB | Methanocaldococcus jannaschii | Tyrosyl-tRNA synthetase,TyrRS |
2 | A | 1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE | non-polymer | 162.2 | 1 | Chemie (P4G) | |||
3 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
4 | A | 4-{[(1R,4E)-cyclooct-4-en-1-ylcarbonyl]amino}-L-phenylalanine | non-polymer | 316.4 | 1 | Chemie (2LQ) | |||
5 | water | water | 18.0 | 210 | Chemie (HOH) |
Sequence modifications
A: 1 - 306 (UniProt: Q57834)
PDB | External Database | Details |
---|---|---|
Gly 32 | Tyr 32 | engineered mutation |
Glu 65 | Leu 65 | engineered mutation |
Trp 108 | Phe 108 | engineered mutation |
Met 109 | Gln 109 | engineered mutation |
Ser 158 | Asp 158 | engineered mutation |
Lys 162 | Leu 162 | engineered mutation |
Leu 307 | - | expression tag |
Glu 308 | - | expression tag |
His 309 | - | expression tag |
His 310 | - | expression tag |
His 311 | - | expression tag |
His 312 | - | expression tag |
His 313 | - | expression tag |
His 314 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 36113.9 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 570.7 | |
All* | Total formula weight | 36684.6 |