4P5O

Structure of an RBX1-UBC12~NEDD8-CUL1-DCN1 complex: a RING-E3-E2~ubiquitin-like protein-substrate intermediate trapped in action

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, C)	Cullin-1	polymer	368	42722.1	2	UniProt (Q13616) Pfam (PF00888) Pfam (PF10557)	Homo sapiens (Human)	CUL-1
2	B, G (B, D)	E3 ubiquitin-protein ligase RBX1	polymer	106	12089.7	2	UniProt (P62877) Pfam (PF12678)	Homo sapiens (Human)	Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1
3	C, H (E, F)	DCN1-like protein 1	polymer	200	23237.5	2	UniProt (Q96GG9) Pfam (PF03556)	Homo sapiens (Human)	DCUN1 domain-containing protein 1,Defective in cullin neddylation protein 1-like protein 1,Squamous cell carcinoma-related oncogene
4	D, I (I, G)	NEDD8-conjugating enzyme Ubc12	polymer	189	21754.8	2	UniProt (P61081) Pfam (PF00179)	Homo sapiens (Human)	NEDD8 carrier protein,NEDD8 protein ligase,Ubiquitin-conjugating enzyme E2 M
5	E, J (K, H)	NEDD8	polymer	81	8919.3	2	UniProt (Q15843) Pfam (PF00240)	Homo sapiens (Human)	Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8
6	K, L, M, N, O... (B, D)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

I, G: 2 - 183 (UniProt: P61081)

PDB	External Database	Details
Ame 1	-	expression tag
Ser 103	Asn 103	engineered mutation
Ser 111	Cys 111	engineered mutation
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	217446.5
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	217839.0

*Water molecules are not included.