4OUD

Engineered tyrosyl-tRNA synthetase with the nonstandard amino acid L-4,4-biphenylalanine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tyrosyl-tRNA synthetase	polymer	425	47399.5	1	UniProt (U6N9P2) Pfam (PF00579) Pfam (PF01479) UniProt (by SIFTS) (A0A0H2UKY9) In PDB	Escherichia coli
2	B	Tyrosyl-tRNA synthetase	polymer	394	43530.5	1	UniProt (U6N9P2) Pfam (PF00579) UniProt (by SIFTS) (A0A0H2UKZ0) In PDB	Escherichia coli
3	A	TYROSINE	non-polymer		181.2	1	Chemie (TYR)
4		water	water		18.0	57	Chemie (HOH)

Sequence modifications

A: 2 - 424 (UniProt: U6N9P2)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag
Ala 49	Leu 49	engineered mutation
Ala 236	Phe 236	engineered mutation
Ala 260	Trp 260	engineered mutation
Ala 263	Thr 263	engineered mutation
Trp 271	Phe 271	engineered mutation
Gly 275	Phe 275	engineered mutation
Bif 303	Leu 303	engineered mutation

B: 2 - 424 (UniProt: U6N9P2)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag
Ala 49	Leu 49	engineered mutation
Ala 236	Phe 236	engineered mutation
Ala 260	Trp 260	engineered mutation
Ala 263	Thr 263	engineered mutation
Trp 271	Phe 271	engineered mutation
Gly 275	Phe 275	engineered mutation
Bif 303	Leu 303	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	90929.9
Non-Polymers*	Number of molecules	1
	Total formula weight	181.2
All*	Total formula weight	91111.1

*Water molecules are not included.