4ORH

Crystal structure of RNF8 bound to the UBC13/MMS2 heterodimer

Replaces:  4EPO

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, H (A, E, I)	Ubiquitin-conjugating enzyme E2 variant 2	polymer	153	17165.6	3	UniProt (Q15819) Pfam (PF00179)	Homo sapiens (human)	DDVit 1, Enterocyte differentiation-associated factor 1, EDAF-1, Enterocyte differentiation-promoting factor 1, EDPF-1, MMS2 homolog, Vitamin D3-inducible protein
2	B, E, I (B, F, J)	Ubiquitin-conjugating enzyme E2 N	polymer	160	17942.6	3	UniProt (P61088) Pfam (PF00179)	Homo sapiens (human)	Bendless-like ubiquitin-conjugating enzyme, Ubc13, UbcH13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N
3	C, F, G, J, K (C, G, H, K, L)	E3 ubiquitin-protein ligase RNF8	polymer	149	17646.5	5	UniProt (O76064) Pfam (PF13920)	Homo sapiens (human)	hRNF8, RING finger protein 8
4	L, M, N, O, P... (C, G, H, K, L)	ZINC ION	non-polymer		65.4	10	Chemie (ZN)

Sequence modifications

A, E, I: 1 - 145 (UniProt: Q15819)

PDB	External Database	Details
Gly -7	-	expression tag
Pro -6	-	expression tag
Leu -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

B, F, J: 1 - 152 (UniProt: P61088)

PDB	External Database	Details
Gly -7	-	expression tag
Pro -6	-	expression tag
Leu -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

C, G, H, K, L: 345 - 485 (UniProt: O76064)

PDB	External Database	Details
Gly 337	-	expression tag
Pro 338	-	expression tag
Leu 339	-	expression tag
Gly 340	-	expression tag
Ser 341	-	expression tag
Pro 342	-	expression tag
Glu 343	-	expression tag
Phe 344	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	193557.1
Non-Polymers*	Number of molecules	10
	Total formula weight	654.1
All*	Total formula weight	194211.2

*Water molecules are not included.