4ORA

Crystal structure of a human calcineurin mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform	polymer	544	61294.5	1	UniProt (P16298) Pfam (PF00149)	Homo sapiens (human)	CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit beta isoform
2	B (B)	Calcineurin subunit B type 1	polymer	170	19322.9	1	UniProt (P63098) Pfam (PF13499)	Homo sapiens (human)	Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D (A)	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
5	E, F, G, H (B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	I, J (A, B)	water	water		18.0	45	Chemie (HOH)

Sequence modifications

A: 1 - 524 (UniProt: P16298)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Tyr 418	Val 418	engineered mutation
Leu 419	Phe 419	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80617.4
Non-Polymers*	Number of molecules	6
	Total formula weight	281.6
All*	Total formula weight	80899.0

*Water molecules are not included.