4OPQ
Room temperature crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying R164S/G238S mutations
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TEM-94 ES-beta-lactamase | polymer | 263 | 28730.7 | 1 | UniProt (Q8KMX3) Pfam (PF00144) Pfam (PF13354) | Escherichia coli | |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 4 | D, E (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 5 | F (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 6 | G (A) | water | water | 18.0 | 130 | Chemie (HOH) |
Sequence modifications
A: 25 - 287 (UniProt: Q8KMX3)
| PDB | External Database | Details |
|---|---|---|
| Gly 41 | Ala 40 | engineered mutation |
| Ala 51 | Asn 50 | engineered mutation |
| Glu 103 | Lys 102 | engineered mutation |
| Gly 119 | Arg 118 | engineered mutation |
| Ser 163 | Arg 162 | engineered mutation |
| Val 183 | Ala 182 | engineered mutation |
| Ala 200 | Leu 199 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28730.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 542.6 | |
| All* | Total formula weight | 29273.3 |
