4OKD

Crystal Structure of Chlamydomonas reinhardtii Isoamylase 1 (ISA1) in complex with maltoheptaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Isoamylase	polymer	840	93054.7	2	UniProt (Q7X8Q2) Pfam (PF02922) Pfam (PF00128) Pfam (PF21156)	Chlamydomonas reinhardtii	Chlamydomonas reinhardtii ISA1
2	C, F (C, F)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		1153.0	2	In PDB GlyTouCan (G41288IQ)
3	D, E, G (D, E, G)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	3	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
4	H (A)	alpha-D-glucopyranose	non-polymer		180.2	1	Chemie (GLC)
5	I, J (A, B)	water	water		18.0	433	Chemie (HOH)

Sequence modifications

A, B: 57 - 875 (UniProt: Q7X8Q2)

PDB	External Database	Details
Met 36	-	expression tag
Gly 37	-	expression tag
Ser 38	-	expression tag
Ser 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
His 45	-	expression tag
Ser 46	-	expression tag
Ser 47	-	expression tag
Gly 48	-	expression tag
Leu 49	-	expression tag
Val 50	-	expression tag
Pro 51	-	expression tag
Arg 52	-	expression tag
Gly 53	-	expression tag
Ser 54	-	expression tag
His 55	-	expression tag
Met 56	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	186109.3
Branched	Number of molecules	5
	Total formula weight	3819.3
Non-Polymers*	Number of molecules	1
	Total formula weight	180.2
All*	Total formula weight	190108.8

*Water molecules are not included.