4OJ0

mCardinal V218E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Fluorescent protein FP480	polymer	246	27721.6	4	UniProt (D0VX33) Pfam (PF01353)	Entacmaea quadricolor (Bubble-tip anemone)
2	E, F, G, H (A, B, C, D)	water	water		18.0	631	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 233 (UniProt: D0VX33)

PDB	External Database	Details
Asp -5	-	expression tag
Pro -4	-	expression tag
Met -3	-	expression tag
Val -2	-	expression tag
Ser -1	-	expression tag
Lys 0	-	expression tag
Gly 1	-	expression tag
Glu 2	-	expression tag
Lys 6	Thr 6	conflict
Pro 10	His 10	conflict
Thr 28	Ser 28	conflict
Cys 61	Ser 61	conflict
Nrq 63	Met 63	chromophore
Nrq 63	Tyr 64	chromophore
Nrq 63	Gly 65	chromophore
Lys 67	His 67	conflict
Lys 71	Asn 71	conflict
Pro 73	Thr 73	conflict
Lys 74	Gln 74	conflict
Phe 80	Trp 80	conflict
Val 104	Ala 104	conflict
Leu 121	Ile 121	conflict
Thr 143	His 143	conflict
Thr 146	Met 146	conflict
Cys 158	Ala 158	conflict
Met 160	Leu 160	conflict
Asp 165	Val 165	conflict
His 171	Ile 171	conflict
Leu 174	Phe 174	conflict
Gly 185	Lys 185	conflict
Phe 194	Tyr 194	conflict
Arg 197	Tyr 197	conflict
Asn 207	Lys 207	conflict
Glu 218	Val 218	engineered mutation
Gly 234	-	expression tag
Met 235	-	expression tag
Asp 236	-	expression tag
Glu 237	-	expression tag
Leu 238	-	expression tag
Tyr 239	-	expression tag
Lys 240	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	110886.3
All*	Total formula weight	110886.3

*Water molecules are not included.