4OHB

Crystal structure of MilB E103A in complex with 5-hydroxymethylcytidine 5'-monophosphate (hmCMP) from Streptomyces rimofaciens

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CMP/hydroxymethyl CMP hydrolase	polymer	190	20470.0	1	UniProt (B4Y381) Pfam (PF05014) In PDB	Streptomyces rimofaciens
2	A	5-(hydroxymethyl)cytidine 5'-(dihydrogen phosphate)	non-polymer		353.2	1	Chemie (5HM)
3		water	water		18.0	37	Chemie (HOH)

Sequence modifications

A: 1 - 170 (UniProt: B4Y381)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 103	Glu 103	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	20470.0
Non-Polymers*	Number of molecules	1
	Total formula weight	353.2
All*	Total formula weight	20823.2

*Water molecules are not included.