4O8T

Structure of sortase A C207A mutant from Streptococcus pneumoniae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Sortase	polymer	187	21077.8	6	UniProt (Q8DPM3) Pfam (PF04203)	Streptococcus pneumoniae
2	G (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	H, I, J, K, L... (A, B, C, D, E...)	water	water		18.0	234	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 82 - 247 (UniProt: Q8DPM3)

PDB	External Database	Details
Met 61	-	expression tag
Gly 62	-	expression tag
Ser 63	-	expression tag
Ser 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag
Ser 71	-	expression tag
Ser 72	-	expression tag
Gly 73	-	expression tag
Leu 74	-	expression tag
Val 75	-	expression tag
Pro 76	-	expression tag
Arg 77	-	expression tag
Gly 78	-	expression tag
Ser 79	-	expression tag
His 80	-	expression tag
Met 81	-	expression tag
Ala 176	Thr 176	engineered mutation
Ala 207	Cys 207	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	126466.7
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	126558.8

*Water molecules are not included.