4O8C

Structure of the H170Y mutant of thermostable p-nitrophenylphosphatase from Bacillus Stearothermophilus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Thermostable NPPase	polymer	261	29139.1	2	UniProt (Q8L1N9) Pfam (PF13344) Pfam (PF13242)	Geobacillus stearothermophilus	thermostable p-nitrophenylphosphatase
2	C (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	D (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	E, F (A, B)	water	water		18.0	258	Chemie (HOH)

Sequence modifications

A, B: 1 - 255 (UniProt: Q8L1N9)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Tyr 170	His 170	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58278.3
Non-Polymers*	Number of molecules	2
	Total formula weight	120.4
All*	Total formula weight	58398.6

*Water molecules are not included.