4O8C
Structure of the H170Y mutant of thermostable p-nitrophenylphosphatase from Bacillus Stearothermophilus
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Thermostable NPPase | polymer | 261 | 29139.1 | 2 | UniProt (Q8L1N9) Pfam (PF13344) Pfam (PF13242) In PDB | Geobacillus stearothermophilus | thermostable p-nitrophenylphosphatase |
2 | B | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
3 | B | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
4 | water | water | 18.0 | 258 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 255 (UniProt: Q8L1N9)
PDB | External Database | Details |
---|---|---|
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
Tyr 170 | His 170 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 58278.3 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 120.4 | |
All* | Total formula weight | 58398.6 |