4O27

Crystal structure of MST3-MO25 complex with WIF motif

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Calcium-binding protein 39	polymer	325	38053.8	1	UniProt (Q9Y376) Pfam (PF08569) In PDB	Homo sapiens (human)	MO25alpha, Protein Mo25
2	B	Serine/threonine-protein kinase 24	polymer	281	31965.7	1	UniProt (Q9Y6E0) Pfam (PF00069) In PDB	Homo sapiens (human)	Mammalian STE20-like protein kinase 3, MST-3, STE20-like kinase MST3, Serine/threonine-protein kinase 24 36 kDa subunit, Mammalian STE20-like protein kinase 3 N-terminal, MST3/N, Serine/threonine-protein kinase 24 12 kDa subunit, Mammalian STE20-like protein kinase 3 C-terminal, MST3/C
3	C	5-mer peptide from serine/threonine-protein kinase 24	polymer	5	708.8	1	UniProt (Q9Y6E0) In PDB
4	B	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5	B	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)

Sequence modifications

A: 11 - 333 (UniProt: Q9Y376)

PDB	External Database	Details
Met 9	-	expression tag
Ala 10	-	expression tag

B: 18 - 297 (UniProt: Q9Y6E0)

PDB	External Database	Details
Met 17	-	expression tag
Glu 178	Thr 190	engineered mutation

C: 1 - 5 (UniProt: Q9Y6E0)

PDB	External Database	Details
Glu 5	Thr 340	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	70728.3
Non-Polymers*	Number of molecules	2
	Total formula weight	482.1
All*	Total formula weight	71210.4

*Water molecules are not included.