4NSV

Lysobacter enzymogenes lysc endoproteinase K30R mutant covalently inhibited by TLCK

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lysyl endopeptidase	polymer	275	28749.2	2	UniProt (Q7M135) Pfam (PF13365) In PDB	Lysobacter enzymogenes	Lys-C
2	A, B	N-[(2S,3S)-7-amino-1-chloro-2-hydroxyheptan-3-yl]-4-methylbenzenesulfonamide (Bound Form)	non-polymer		334.9	2	BIRD (PRD_001217) Chemie (2OY)
3	A, B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	937	Chemie (HOH)

Sequence modifications

A, B: 1 - 266 (UniProt: Q7M135)

PDB	External Database	Details
Arg 30	Lys 30	engineered mutation
Gly 267	-	expression tag
Ser 268	-	expression tag
Gly 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57498.4
Non-Polymers*	Number of molecules	6
	Total formula weight	993.4
All*	Total formula weight	58491.8

*Water molecules are not included.