4NRI

Crystal Structure of a human Mms2/Ubc13 A122G mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin-conjugating enzyme E2 variant 2	polymer	148	16676.0	1	UniProt (Q15819) Pfam (PF00179) In PDB	Homo sapiens (human)	DDVit 1, Enterocyte differentiation-associated factor 1, EDAF-1, Enterocyte differentiation-promoting factor 1, EDPF-1, MMS2 homolog, Vitamin D3-inducible protein
2	B	Ubiquitin-conjugating enzyme E2 N	polymer	156	17499.1	1	UniProt (P61088) Pfam (PF00179) In PDB	Homo sapiens (human)	Bendless-like ubiquitin-conjugating enzyme, Ubc13, UbcH13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	82	Chemie (HOH)

Sequence modifications

A: 6 - 145 (UniProt: Q15819)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Leu 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Pro 3	-	expression tag
Glu 4	-	expression tag
Phe 5	-	expression tag

B: 3 - 150 (UniProt: P61088)

PDB	External Database	Details
Gly -5	-	expression tag
Pro -4	-	expression tag
Leu -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Pro 0	-	expression tag
Glu 1	-	expression tag
Phe 2	-	expression tag
Gly 122	Ala 122	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	34175.1
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	34267.2

*Water molecules are not included.