4NQS

Knob-into-hole IgG Fc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, G)	Ig gamma-1 chain C region	polymer	213	24000.1	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (human)	hole Fc T366S/L368A/Y407V
2	B, F (B, H)	Ig gamma-1 chain C region	polymer	213	24205.4	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (human)	knob Fc T366W
3	C, D, G, H (D, E, I, J)	miniZ	polymer	34	4190.7	4		Staphylococcus
4	I, J, K, L, M... (A, B, D, G, H...)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, G: 235 - 447 (UniProt: P01857)

PDB	External Database	Details
Glu 356	Asp 239	SEE REMARK 999
Met 358	Leu 241	SEE REMARK 999
Ser 366	Thr 249	engineered mutation
Ala 368	Leu 251	engineered mutation
Val 407	Tyr 290	engineered mutation

B, H: 235 - 447 (UniProt: P01857)

PDB	External Database	Details
Glu 356	Asp 239	SEE REMARK 999
Met 358	Leu 241	SEE REMARK 999
Trp 366	Thr 249	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	113173.8
All*	Total formula weight	113173.8

*Water molecules are not included.