4NJE

Crystal structure of Pyrococcus furiosus L-asparaginase with ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	L-asparaginase	polymer	204	22265.5	1	UniProt (Q8TZE8) Pfam (PF00710) In PDB	Pyrococcus furiosus
2	B	L-asparaginase	polymer	147	16075.7	1	UniProt (Q8TZE8) Pfam (PF17763) In PDB	Pyrococcus furiosus
3	A	ASPARTIC ACID	non-polymer		133.1	1	Chemie (ASP)
4		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A: 1 - 182 (UniProt: Q8TZE8)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

B: 202 - 326 (UniProt: Q8TZE8)

PDB	External Database	Details
Met 180	-	expression tag
Gly 181	-	expression tag
Ser 182	-	expression tag
Ser 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
Ser 190	-	expression tag
Ser 191	-	expression tag
Gly 192	-	expression tag
Leu 193	-	expression tag
Val 194	-	expression tag
Pro 195	-	expression tag
Arg 196	-	expression tag
Gly 197	-	expression tag
Ser 198	-	expression tag
His 199	-	expression tag
Met 200	-	expression tag
Ala 201	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38341.2
Non-Polymers*	Number of molecules	1
	Total formula weight	133.1
All*	Total formula weight	38474.3

*Water molecules are not included.