4NIV
Crystal structure of trypsiligase (K60E/N143H/Y151H/D189K trypsin) trigonal form
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cationic trypsin | polymer | 223 | 23337.3 | 1 | UniProt (P00760) Pfam (PF00089) | Bos taurus (bovine cow domestic cattle domestic cow) | Beta-trypsin, Alpha-trypsin chain 1, Alpha-trypsin chain 2 |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | D (A) | water | water | 18.0 | 185 | Chemie (HOH) |
Sequence modifications
A: 16 - 245 (UniProt: P00760)
| PDB | External Database | Details |
|---|---|---|
| Glu 60 | Lys 66 | engineered mutation |
| His 143 | Asn 146 | engineered mutation |
| His 151 | Tyr 154 | engineered mutation |
| Lys 189 | Asp 194 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 23337.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 132.2 | |
| All* | Total formula weight | 23469.5 |
