4NIH
Crystal structure of AlkB E136L mutant protein with cofactors bound to dsDNA containing m6A/A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Alpha-ketoglutarate-dependent dioxygenase AlkB | polymer | 205 | 22814.1 | 1 | UniProt (P05050) In PDB | Escherichia coli | Alkylated DNA repair protein AlkB, DNA oxidative demethylase AlkB |
| 2 | B | 5'-D(*TP*AP*GP*GP*TP*AP*AP*(6MA)P*AP*CP*CP*GP*T)-3' | polymer | 13 | 4073.8 | 1 | |||
| 3 | C | 5'-D(*AP*AP*CP*GP*GP*TP*AP*TP*TP*AP*CP*CP*T)-3' | polymer | 13 | 3950.6 | 1 | |||
| 4 | A | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 5 | A | 2-OXOGLUTARIC ACID | non-polymer | 146.1 | 1 | Chemie (AKG) | |||
| 6 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | water | water | 18.0 | 472 | Chemie (HOH) |
Sequence modifications
A: 12 - 216 (UniProt: P05050)
| PDB | External Database | Details |
|---|---|---|
| Cys 129 | Ser 129 | engineered mutation |
| Leu 136 | Glu 136 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 30838.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 293.1 | |
| All* | Total formula weight | 31131.7 |
