4NF8

Crystal structure of GluN1/GluN2A ligand-binding domain in complex with glycine and glutamate in PEG2000MME

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glutamate receptor ionotropic, NMDA 1	polymer	292	33340.0	1	UniProt (P35439) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (rat)	GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1
2	B (B)	Glutamate receptor ionotropic, NMDA 2A	polymer	283	31785.3	1	UniProt (Q00959) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (rat)
3	C (A)	GLYCINE	non-polymer		75.1	1	Chemie (GLY)
4	D (B)	GLUTAMIC ACID	non-polymer		147.1	1	Chemie (GLU)
5	E, F (A, B)	water	water		18.0	593	Chemie (HOH)

Sequence modifications

A: 2 - 152 (UniProt: P35439)

PDB	External Database	Details
Gly 1	-	expression tag

A: 155 - 292 (UniProt: P35439)

PDB	External Database	Details
Gly 153	-	expression tag
Thr 154	-	expression tag

B: 5 - 142 (UniProt: Q00959)

PDB	External Database	Details
Ser 4	-	expression tag

B: 145 - 286 (UniProt: Q00959)

PDB	External Database	Details
Thr 242	Ser 758	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65125.3
Non-Polymers*	Number of molecules	2
	Total formula weight	222.2
All*	Total formula weight	65347.5

*Water molecules are not included.